1Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 2School of Biology, Georgia Institute of Technology
Thursday All day, Plaza Level
The genome sequences of several primate species are available, providing insights into evolutionary and anthropological research. However, obtaining large amounts of sequence data for species whose genomes are not yet available remains time consuming and costly. Within primates genomic resources from platyrrhines are especially lacking, to date the genome of only the common marmoset, has been sequenced. This is especially limiting, considering that platyrrhines are the most speciose primate group, whose genetic diversity is comparable to that of the Catarrhini.
Here we present genomic markers developed from Spider monkey (Ateles belzebuth), Owl monkey (Aotus lemurinus), and Bald Uakari (Cacajao calvus) representing the three platyrrhine families, Atelidae, Cebidae, and Pitheciidae respectively. Markers were developed from gDNA shotgun libraries containing >3,000 individual sequences with a mean length of 736 bps. Among them a large number of markers were found to have unique hits within the human (n=2,179) and the marmoset (n=2,520) genomes. Of these sequences, 970 contain <20% repeats, and are potentially highly useful markers in other platyrrhines. We determined the relative location of these markers in orthologous genomes is relation to exons, introns, and intergenic regions. > 90% of these marker sequences are located in non-coding DNA. Thus, they are likely highly useful for inferring neutral evolutionary patterns and genomic evolution in related platyrrhine species.
These annotated marker sequences are publicly available for use and will be a valuable resource for future phylogenetic and population studies as they currently provide the largest single resource of molecular data on these species.
This study was funded by National Science Foundation grants BCS-0751508 and BCS-0751481. NMJ is funded by the National Science Foundation grant BCS-1061370