The 82nd Annual Meeting of the American Association of Physical Anthropologists (2013)


Ancient tuberculosis DNA revealed by Next Generation Sequencing

ABIGAIL S. BOUWMAN1,3, SANDRA KENNEDY1, ROMY MUELLER1, CHARLOTTE ROBERTS2 and TERRY BROWN1.

1Faculty of Life Sciences, University of Manchester, 2Department of Archarology, Durham University, 3Centre for Evolutionary Medicine, University of Z├╝rich

Thursday 9:45-10:00, Ballroom A Add to calendar

Mycobacterium tuberculosis complex bacteria were the first pathogens targeted in ancient DNA studies, and throughout the period that ancient bacterial DNA has been studied, has had a very constant presence. As the field of aDNA has progressed and more technologies have become available, the research into the causative agent of tuberculosis has been able to become more in-depth. A project was undertaken by the Universities of Manchester and Durham to investigate tuberculosis from the Neolithic until the 19th Century CE across the whole of Europe. This project screened over 500 samples from nearly 100 sites for the presence of Mycobacterium tuberculosis complex DNA before sending 101 samples for targeted next generation sequencing. Here we present the background of ancient tuberculosis research, from the beginnings to today, before detailing the results of the study in the study in comparison to the modern data. One individual from 19th Century Leeds was typed with 218 SNPs, eight in/dels and two insertion sequences using SOLiD sequencing and was found to be closely related, but not identical, to the H37Rv strain isolated in 1905.

Please note, AB is now at the University of Zurich, but all work was undertaken at the University of Manchester. Work Funded by NERC.

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